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Antibody Conjugation Services
Move quickly from idea to experiment with antibody conjugation services that are typically complete within
5 - 10 days.
Whether you need an alternative conjugate of a SouthernBiotech-manufactured antibody or a custom conjugation of your own antibody, our team of protein chemists will use our proprietary processes to deliver reagents that drive your success.
Service Overview
The expert team at SouthernBiotech can provide antibody conjugation services with over 20 fluorochrome and enzyme labeling options, affordable pricing, fast turnaround times, and scalable processes from milligrams to grams.
We have spent over three decades providing antibody conjugation services to the academic, biopharma, and diagnostic communities, and in that time we have developed proprietary labeling and post-conjugation purification processes that maximize signal to noise ratios. The result is a highly efficient workflow that delivers outstanding performance for ELISA, immunohistochemistry (IHC) assays, flow cytometry, western blot, and more.
Expert technical support
Customizable to suit your needs
100% in-house production
Custom Antibody Conjugation Process
Choose a SouthernBiotech-manufactured antibody or send us your antibody—we can conjugate it to any of the molecules listed below as well as custom molecules.
Antibody Conjugation Protocols
| Conjugate | Excitation Max (nm) | Emission Max (nm) | Emission Color |
|---|---|---|---|
| Alexa Fluor® 488 (AF488) | 490 | 525 | Green |
| Alexa Fluor® 555 (AF555) | 555 | 580 | Orange |
| Alexa Fluor® 647 (AF647) | 650 | 665 | Red |
| Alexa Fluor® 700 (AF700) | 702 | 723 | Near IR |
| Alkaline Phosphatase (AP) | Â | Â | Â |
| Allophycocyanin (APC) | 652 | 658 | Red |
| Allophycocyanin/Cyanine 5.5 (APC/CY5.5) | 652 | 700 | Near IR |
| Allophycocyanin/Cyanine 7 (APC/CY7) | 652 | 774 | Near IR |
| β-galactosidase (BGAL) |  |  |  |
| Biotin (BIOT) | Â | Â | Â |
| Cyanine 3 (CY3) | 550 | 570 | Orange |
| Cyanine 5 (CY5) | 650 | 670 | Red |
| Fluorescein Isothiocyanate (FITC) | 492 | 518 | Green |
| Horseradish Peroxidase (HRP) | Â | Â | Â |
| Pacific Blueâ„¢ (PACBLU) | 410 | 455 | Blue |
| R-Phycoerythrin (PE) | 566 (496) | 575 | Orange |
| R-Phycoerythrin/Cyanine 5.5 (PE/CY5.5) | 566 (496) | 690 | Red |
| R-Phycoerythrin/Cyanine 7 (PE/CY7) | 566 (496) | 774 | Near IR |
| R-Phycoerythrin/Texas Red® (PE/TXRD) | 566 (496) | 615 | Red |
| Sepharose® (SEPH) |  |  |  |
| Spectral Red® (SPRD) | 566 (496) | 670 | Red |
| Tetramethylrhodamine Isothiocyanate (TRITC) | 555 | 580 | Orange |
| Texas Red® (TXRD) | 595 | 615 | Red |
FAQs
We typically recommend starting with ≥ 2 mg; however, depending on the conjugate, we may be able accommodate smaller quantities.
The minimum concentration required will be dependent on the conjugate; however, we do offer custom concentration services, if required.
Antibodies should be supplied in a neutral buffer (e.g., PBS) with no stabilizers, preservatives, amine containing buffer salts, etc. If needed, we offer custom buffer exchange services.
Most custom conjugated antibodies are available for shipment within 5 - 10 business days following our receipt of your antibody and purchase order.
Initiate a Custom Service Project
Proven expertise, outstanding service, and responsive customer support are just a few of the reasons why scientists continue to turn to SouthernBiotech for antibody conjugation services.
Start an antibody conjugation project with us and experience the SouthernBiotech difference for yourself.
