Antibody Conjugation Services

Move quickly from idea to experiment with antibody conjugation services that are typically complete within
5 - 10 days.
Whether you need an alternative conjugate of a SouthernBiotech-manufactured antibody or a custom conjugation of your own antibody, our team of protein chemists will use our proprietary processes to deliver reagents that drive your success.

Service Overview

The expert team at SouthernBiotech can provide antibody conjugation services with over 20 fluorochrome and enzyme labeling options, affordable pricing, fast turnaround times, and scalable processes from milligrams to grams.

We have spent over three decades providing antibody conjugation services to the academic, biopharma, and diagnostic communities, and in that time we have developed proprietary labeling and post-conjugation purification processes that maximize signal to noise ratios. The result is a highly efficient workflow that delivers outstanding performance for ELISA, immunohistochemistry (IHC) assays, flow cytometry, western blot, and more.

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Expert technical support

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Customizable to suit your needs

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100% in-house production

Custom Antibody Conjugation Process

Choose a SouthernBiotech-manufactured antibody or send us your antibody—we can conjugate it to any of the molecules listed below as well as custom molecules.

Antibody Conjugation Protocols

Conjugate Excitation Max (nm) Emission Max (nm) Emission Color
Alexa Fluor® 488 (AF488) 490 525 Green
Alexa Fluor® 555 (AF555) 555 580 Orange
Alexa Fluor® 647 (AF647) 650 665 Red
Alexa Fluor® 700 (AF700) 702 723 Near IR
Alkaline Phosphatase (AP)      
Allophycocyanin (APC) 652 658 Red
Allophycocyanin/Cyanine 5.5 (APC/CY5.5) 652 700 Near IR
Allophycocyanin/Cyanine 7 (APC/CY7) 652 774 Near IR
β-galactosidase (BGAL)      
Biotin (BIOT)      
Cyanine 3 (CY3) 550 570 Orange
Cyanine 5 (CY5) 650 670 Red
Fluorescein Isothiocyanate (FITC) 492 518 Green
Horseradish Peroxidase (HRP)      
Pacific Blue™ (PACBLU) 410 455 Blue
R-Phycoerythrin (PE) 566 (496) 575 Orange
R-Phycoerythrin/Cyanine 5.5 (PE/CY5.5) 566 (496) 690 Red
R-Phycoerythrin/Cyanine 7 (PE/CY7) 566 (496) 774 Near IR
R-Phycoerythrin/Texas Red® (PE/TXRD) 566 (496) 615 Red
Sepharose® (SEPH)      
Spectral Red® (SPRD) 566 (496) 670 Red
Tetramethylrhodamine Isothiocyanate (TRITC) 555 580 Orange
Texas Red® (TXRD) 595 615 Red

FAQs

We typically recommend starting with ≥ 2 mg; however, depending on the conjugate, we may be able accommodate smaller quantities.

The minimum concentration required will be dependent on the conjugate; however, we do offer custom concentration services, if required.

Antibodies should be supplied in a neutral buffer (e.g., PBS) with no stabilizers, preservatives, amine containing buffer salts, etc. If needed, we offer custom buffer exchange services.

Most custom conjugated antibodies are available for shipment within 5 - 10 business days following our receipt of your antibody and purchase order.

Initiate a Custom Service Project

Proven expertise, outstanding service, and responsive customer support are just a few of the reasons why scientists continue to turn to SouthernBiotech for antibody conjugation services.

Start an antibody conjugation project with us and experience the SouthernBiotech difference for yourself.